Fautrel Alain

  • Ph.D. - Ingénieur de recherche
  • Inserm
  • H2P2 - Histo-pathologie
    laboratoire d'anatomie pathologique
    faculté de Médecine
    2 Avenue du professeur Léon Bernard
    35043 Rennes Cedex

  • Téléphone : 0223234795
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  • Publié par : Alain Fautrel
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English presentation

The H2P2 plateform is more than a core facility in the field of histopathology. H2P2 also boasts hightech equipment such as the discovery XT biomarker platform (Ventana), the veritas laser capture microdissector (Arcturus), the TMA automated tissuarrayer (Excilone), the nanozoomer virtual slide scanner aquipped with Fluorescence illumination Optics (Hamamatsu), NIS elements image analysis software (Nikon): measurement of immunochemistry/fluorescence, cell shape/size/volume, cell counting, specific staining, etc. These facilities enable us to meet the requirements of research teams in the field of in vivo toxicity and pharmacology, biomarker research for human diseases, as well as animal and plant research.

 

Expertise focus

Our level of expertise on tissue damage, detected using a range of specific staining and immunostaining or fluorescence methods, enables quantitative measurement by image analysis of specific markers by our hignly experienced team. We are supported by the pathologyst at Rennes University hospital. We also have extensive experience in the laser capture microdissection of numerous animal and plant species. Following specific cell capture we achieve the RNA quality required for RT-qPCR or transcriptomics.

Routine and specific tests

Quantitative measurement:

   - immuno-histochemistry quantification: apoptosis, proliferation, expression of interest proteins.

   - specific staining : quantification of collagen, iron, mucus secretion, glycogen, etc.

exemples:

   - lung : emphysema, fibrosis, inflammation

   - liver: fibrosis, steatosis, necrosis

   - intestine : vilosity measurement, inflammation, mucus secretion

   - skin : apoptosis, proliferation

Gene expression in different cells types, in the sme tissue by laser microdissection (qPCR or transcriptomique) or hybridation in situ.

Accreditation - certification

The core facility has taken a Quality management approach and obtained  the ISO 9001 certification in June 2012